Volume 6.10 | Mar 17

Pancreatic Cell News 6.10 March 17, 2015
Pancreatic Cell News
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Weaning Triggers a Maturation Step of Pancreatic β Cells
Researchers examined the effect of young age on compensatory proliferation of pancreatic β cells in vivo. Surprisingly, β cells in suckling mice fail to enter the cell division cycle in response to a diabetogenic injury or increased glycolysis. [Dev Cell] Abstract | Press Release
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PUBLICATIONS (Ranked by impact factor of the journal)

Characterization of Pancreatic NMDA Receptors as Possible Drug Targets for Diabetes Treatment
Researchers found that inhibition of NMDA receptors (NMDARs) in mouse and human islets enhanced their glucose-stimulated insulin secretion and survival of islet cells. Further, NMDAR inhibition prolonged the amount of time that glucose-stimulated beta cells spent in a depolarized state with high cytosolic Ca2+ concentrations. [Nat Med] Abstract | Press Release

A Live-Cell, High-Content Imaging Survey of 206 Endogenous Factors across Five Stress Conditions Reveals Context-Dependent Survival Effects in Mouse Primary Beta Cells
Primary mouse islet cells were cultured in conditions mimicking five type 1 or type 2 diabetes-related stresses: basal 5 mmol/L glucose, cytokine cocktail, 1 μmol/L thapsigargin, 1.5 mmol/L palmitate and 20 mmol/L glucose. Scientists surveyed the effects of a library of 206 endogenous factors on islet cell survival through multi-parameter, live-cell imaging. [Diabetologia] Full Article

Unique and Shared Metabolic Regulation in Clonal β-Cells and Primary Islets Derived from Rat Revealed by Metabolomics Analysis
Scientists compared metabolite profiles and gene expression in rat islets and the INS-1 832/13 line, a widely used clonal β-cell model. They found that insulin secretion and metabolic regulation provoked by glucose were qualitatively similar in these β-cell models. [Endocrinology] Abstract

Transcriptional Activity of the Islet β Cell Factor Pdx1 Is Augmented by Lysine Methylation Catalyzed by the Methyltransferase Set7/9
Researchers tested the hypothesis that Lys methylation of Pdx1 by Set7/9 augments Pdx1 transcriptional activity. Cell-based luciferase reporter assays using wild-type and mutant transgenes revealed a requirement of Pdx1 residue K131, but not K123, for transcriptional augmentation by Set7/9. [J Biol Chem]
Abstract | Full Article

Fate Mapping of ptf1a-Expressing Cells during Pancreatic Organogenesis and Regeneration in Zebrafish
To delineate the lineage potential of pancreas transcription factor 1a (ptf1a)-expressing cells, researchers generated ptf1a:creERT2 transgenic fish and performed genetic-inducible lineage tracing in developmental, regenerating, and ptf1a-deficient zebrafish pancreas. [Dev Dyn] Abstract


SIRT3-Dependent GOT2 Acetylation Status Affects the Malate-Aspartate NADH Shuttle Activity and Pancreatic Tumor Growth
Glutamate oxaloacetate transaminase 2 (GOT2) K159, K185, and K404 (3K) acetylation stimulates NADPH production to suppress ROS and to protect cells from oxidative damage. Moreover, GOT2 3K acetylation promotes pancreatic cell proliferation and tumor growth in vivo. [EMBO J] Abstract | Graphical Abstract

RSpo2 Enhances Canonical Wnt Signaling to Confer Stemness-Associated Traits to Susceptible Pancreatic Cancer Cells
Researchers describe small subpopulations of pancreatic cancer cells with high intrinsic Wnt activity that possess properties indicative of cancer stem cells, including drug-resistance and tumor initiating capacity, while cell populations with negligible Wnt activity preferentially express markers of differentiation. [Cancer Res] Abstract

Embigin Is Overexpressed in Pancreatic Ductal Adenocarcinoma and Regulates Cell Motility through Epithelial to Mesenchymal Transition via the TGF-β Pathway
Scientists transfected BxPC-3 and HPAC pancreatic cancer cells with siRNA or shRNA targeting Embigin and observed reductions in cell proliferation, migration, invasion, wound healing, and reduced levels of matrix metalloproteinases-2 and -9. [Mol Carcinog] Abstract

Oncotropic H-1 Parvovirus Infection Degrades HIF-1α Protein in Human Pancreatic Cancer Cells Independently of VHL and RACK1
Researchers found that infection with the oncolytic H-1 parvovirus decreased HIF-1α protein levels in pancreatic cancer cells under CoCl2 or hypoxia. [Int J Oncol] Abstract

Aberrant Over-Expression of TRPM7 Ion Channels in Pancreatic Cancer: Required for Cancer Cell Invasion and Implicated in Tumor Growth and Metastasis
Researchers determined the expression levels of transient receptor potential melastatin-subfamily member 7 (TRPM7) in pancreatic tissue microarrays and correlated these measurements in pancreatic adenocarcinoma with the clinicopathological features. They also investigated the role of TRPM7 channels in pancreatic cancer cell invasion using the Matrigel™-coated transwell assay. [Biol Open] Full Article

Free Cell Stem Cell Poster: Directed Differentiation of ESCs/iPSCs
The Role of p21-Activated Kinases in Pancreatic Cancer
The authors highlight the potential role of p21-activated kinases in pancreatic cancer and provides a foundation for more effective therapeutics to improve our current treatment of pancreatic cancer. [Pancreas] Abstract

Visit our reviews page to see a complete list of reviews in the pancreatic cell research field.
ViaCyte to Present at ARM’s Third Annual Regen Med Investor Day
ViaCyte, Inc. announced a presentation by Dr. Paul Laikind, President and CEO, at the third annual Regen Med Investor Day co-hosted by the Alliance for Regenerative Medicine (ARM). [ViaCyte, Inc.] Press Release

The Alliance for Regenerative Medicine Enters into Memorandum of Understanding with Japan’s Forum for Innovative Regenerative Medicine
The Alliance for Regenerative Medicine announced it has entered into a memorandum of understanding with Japan’s Forum for Innovative Regenerative Medicine, effective immediately. [The Alliance for Regenerative Medicine] Press Release

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NEW 2015 World Stem Cell Summit (WSCS15)
December 10-12, 2015
Atlanta, United States

Visit our events page to see a complete list of events in the pancreatic cell community.
NEW Postdoctoral Positions – Developmental Biology (Joslin Diabetes Center)

Scientist – Pluripotent Stem Cell Biology Endoderm Lineages (STEMCELL Technologies Inc.)

Research Associate – Cell Separation (STEMCELL Technologies Inc.)

Postdoctoral Fellow – Type 2 Diabetes (Harvard Medical School)

PhD Student Positions – Regenerative Medicine/Stem Cell Biology (University Hospital Tubingen)

Tenure-Track Immunology Faculty Position – Diabetes (University of Connecticut Health Center)

Clinical Research Fellow – Pancreatic Cancer Biology (Cancer Research UK)

Postdoctoral Position – Endothelial Dysfunction and Diabetes (Weill Cornell Medical College in Qatar)

Director – Vector Production (Sangamo BioSciences, Inc.)

Senior Process Development Engineer (Sangamo BioSciences, Inc.)

Assistant/Associate Professor – Endocrinology, Diabetes and Metabolism Research (University of Pennsylvania)

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